Effects of plasma acid on rat uterine tissue <i>in vitro</i>

The aim of the study was to evaluate effect plasma acid on uterine tissue laboratory animals in vitro . Materials and methods. Treatment dimethyl sulfoxide – water solution for injections with a spark discharge air resulted decrease pH, which contributed generation solutions. We incubated tissues at room temperature 30 minutes. treated were examined histologically immunohistochemically. Results. showed that had pronounced biological activity. Immunohistochemistry used show that, depending type solution, altered nitrosative damage products (3-NT) oxidative DNA (8-OHdG) modulated number cells high proliferative potential (including CD133 + cells) production vascular endothelial growth factor (VEGF). These effects general cytotoxicity Conclusion. During 30-minute exposure , prepared from (DMSO) mixture exhibits various samples obtained experimental animals. Plasma-treated exerts cytotoxic associated promotes induction pro-angiogenic activity tissue. DMSO does not have effect. It inhibits cell proliferation, reducing population VEGF